The museum’s permanent collection includes over 20,000 works of art such as paintings, photographs, sculptures, prints and drawings. Notable artists in the collection include Ansel Adams, Gustave Baumann, Georgia O'Keeffe, Fritz Scholder, T. C. Cannon, Bruce Nauman, Luis Jimenez, Maria Martinez, members of the Ashcan School, Los Cinco Pintores, Transcendental Painting Group, and the Taos Society of Artists.
The St. Francis Auditorium, located in the New Mexico Museum of Art, is the venue for various cultural and musical organizations, including the Santa Fe Chamber Music Festival and the Santa Fe Community Orchestra. The auditorium has a seating capacity of 450. The auditorium displays several murals depicting St. Francis of Assisi which were originally designed by Donald Beauregard and completed by Carlos Vierra and Kenneth Chapman.
The museum library contains art books, periodicals, biographical files of artists represented in the permanent collection and catalogs of exhibitions since 1917.