This chapel is an example of Mediterranean Revival architecture and is notable for its distinctive campanile, the upper section of the chapel tower. The campanile and other architectural features are heavily inspired by the Cathedral of Toledo in Spain. The chapel bell, which rings every day at noon, is the same one that called students to the first day of class in 1885. Knowles Chapel sits adjacent to the Annie Russell Theater, separated by a chapel garden, and across from a rose garden.
Above the entrance of the chapel is a relief inscribed with the motto of Rollins College, Fiat Lux. Other notable features of the chapel are the stained class windows, including the Rose Window, a circular stained-glass window on which is written Wisdom is Better than Strength. Four of the windows honor individuals instrumental in the founding of the school. Inside the chapel, above the entrance, is a bas relief depicting Jesus, the Virgin Mary, the disciple John, and the words Peace I Leave With You. The altar area is decorated with a hand carving of the Last Supper by Ernest Peligrini.
Additionally, the chapel houses an organ, originally built in 1932 by Ernest M. Skinner. Skinner's company, Aeolian-Skinner, renovated the organ in the 1950s, and the organ was again renovated in from 1999 to 2002, this time by Randall Dyer & Associates. Additionally, Dyer & Associates also installed a free-standing antiphonal organ at the rear balcony.
In 1997, the National Park service added Knowles Chapel to the National Register of Historic Places. The site was also recognized by the Florida chapter of the American Institute of Architects (AIA) in 2012. Weekly Christian services occur at the chapel every Sunday. It also hosts classes on Scripture and Theology, and conducts
marriages, baptisms, funerals and memorial services for members of the
Rollins community. The beauty of Knowles Chapel makes it a popular venue for weddings, music events, and photography.