The museum used to be called the Mount Horeb Mustard Museum; however, in November of 2009, the museum was moved to Middleton and renamed the National Mustard Museum.
A gift shop occupies roughly half of the museum space. The gift shop includes free mustard tasting and the museum operates a mail-order mustard business. Admission is free. The museum has been featured on the Food Network and the National Public Radio's broadcast.
The town of Middleton holds a festival each year on National Mustard Day, which is the first Saturday of every August.2