Located in the historic depot building at the intersection of the two major railroads which was the center of DeQuincy when the town was founded, the DeQuincy Railroad Museum acquires, preserves and displays railroad-related artifacts and memorabilia. Its mission is to educate the public about the town's railroading history as well as that of the the state, region and the rest of the country. Notable artifacts include model trains and Gauge 1 model steam and diesel engines. Outside the building and covered under a pavilion are a 1913 steam engine, a 1947 passenger train and two vintage cabooses. Visitors are offered tours inside these cars. The museum also offers a small library of materials related to railroad and local history. There is also a gift shop located in the smaller waiting room.
The Kansas City Railroad Company built the depot in 1923 in the Mission Revival style. It was typical at that time for stations to be more than one story and aesthetically striking. The depot is one of three historic train stations in Louisiana. The museum was opened in 1974.